To evaluate the relationship between c-myc amplification and leukemic transformation. The Southern hybridization was performed in 23 clinical leukemic samples(4 cases of ALL, 17 cases of ANLL, 1 cases of CML, 1 cases of CMMoL), 6 ATCC
leukemic
cell
lines (ATCC CCL 213 Daudi;Burkitt lymphoma, ATCC CCL 243 k-562;chronic myelogenous leukemia, ATCC CRL 2582 Molt-4; T-cell acute lymphoblastic leukemia, ATCC CCL 246.1 KG-la;acute myeloblastic leukemia), and 6 cases of nonleukemic bone
marrow
aspirates.
@ES The results were summarized as follows.
@EN 1. In leukemic cell lines, HL-60 showed marked c-myc amplification(6X) compared to nogative control and KGl showed mild amplification(2x). However, there was no c-myc amplification in K-562, KG1a, and molt-4.
2. In leukemic samples, mild c-myc amplification(2x) was observed in 3 cases of 17 AML, but no amplification was observed in 4 ALLs, 1 CML and 1 CMMoL.
3. In nonleukemic samples, one case of reactive marrow hyperplasia showed mild c-myc amplification(2x).
From the above findings, it was concluded that the c-myc amplification was more common in acute nonlymphocytic leukemia than other types of leukemias. c-myc amplification appeared not only enough to transform hematopoietic cells but expression
of
other complicated oncogenes together with it cotributed the development of leukemia
|